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What goes on inside a pathology laboratory?

What goes on inside a pathology laboratory?

International Pathology Day is on 8 November 2023. To celebrate, we’ve given some budding young scientists access to one of our newest laboratories to find out what happens behind the lab doors!

Inter­na­tion­al Pathol­o­gy Day is all about cel­e­brat­ing the impor­tance of pathol­o­gy in our health­care system.

Pathol­o­gy plays an invalu­able role in the detec­tion, diag­no­sis, and treat­ment of dis­ease.  As Australia’s largest pub­lic pathol­o­gy provider, we’re proud that our net­work of over 60 lab­o­ra­to­ries and 150+ col­lec­tion cen­tres is keep­ing our com­mu­ni­ty safe and healthy.

This year, we’ve opened our doors to some future sci­en­tists – Spencer and Emil­ia (both aged 7) – to tour our Nepean lab­o­ra­to­ry which was offi­cial­ly opened in August and inter­view some of the staff who work there.

Cather­ine Jan­to is Spencer’s aunt and is the A/Senior Hos­pi­tal Sci­en­tist in Charge of Micro­bi­ol­o­gy at the Nepean laboratory.

She began work­ing in micro­bi­ol­o­gy 13 years ago and says she loves that the job is all about help­ing patients.

A woman wearing a white lab coat sits at a laboratory desk talking to a young boy in a lab coat.
Spencer’s aunt Cather­ine talks him through a day in the life of Nepean pathol­o­gy laboratory.

“We play a big role in diag­nos­ing infec­tions. We grow bac­te­ria, detect virus­es, par­a­sites and fun­gi to work out why some­one is sick, and what kind of infec­tion they may have,” she explained.

“In the lab we don’t see patients face-to-face, we are focused on our agar plates and PCR ampli­fi­ca­tion curves, so it’s good to remind our­selves there are patients who we’re help­ing behind every result.

“It’s a reward­ing feel­ing to be able to work in this brand-new lab­o­ra­to­ry that we designed and got up and run­ning, essen­tial­ly for the future of Nepean Hos­pi­tal and sur­round­ing community.”

A woman in a white lab coat stands next to a young girl in a lab coat holding a microphone.
Michelle Bas­nett explains to her niece Emil­ia why she loves work­ing in pathology.

Michelle Bas­nett, Emilia’s aunt, is a tech­ni­cal offi­cer in the lab and told her niece that even though her job involves study­ing poo – she loves work­ing in pathology!

“We receive all sorts of sam­ples here at the lab, like urine, we look at stools or poo, tis­sue or flesh from the body, swabs, we also look at blood and oth­er flu­id com­ing from the body,” she said.

“I chose to work in pathol­o­gy because I real­ly liked sci­ence at school and I was inter­est­ed in how the body works.

“It’s very hands on and I’m a very visu­al per­son, so I like to use my obser­va­tion­al skills, for exam­ple using the micro­scope to look for organ­isms, I find it all quite interesting.”

Thanks Spencer and Emil­ia for check­ing out the lab and we hope to see you back in front of a micro­scope again one day!

Challenges of testing urine for drugs of abuse

Testing urine for drugs is a well-accepted scientific process, providing verifiable and reliable results. But testing whether the urine is real and not synthetic or adulterated has been more difficult, until now.

Dr Ashraf Mina is a prin­ci­pal sci­en­tist at NSW Health Pathology’s Foren­sic & Ana­lyt­i­cal Sci­ence Ser­vice, based at the Drug Tox­i­col­o­gy Unit, which tests urine sam­ples for evi­dence of drugs, both drugs of abuse and therapeutic.

He says a chance con­ver­sa­tion a cou­ple of years ago got him won­der­ing why there are no defin­i­tive tests to deter­mine that a urine sam­ple is actu­al­ly real human urine.

“There is no stan­dard for it world­wide, there are some attempts to test for the most com­mon para­me­ters in urine, but they can be exact­ly mim­ic­ked in syn­thet­ic urine,” Dr Mina said.

So, he got to work and has devel­oped a nov­el approach to sam­ple valid­i­ty test­ing and he recent­ly pre­sent­ed his find­ings to a research sym­po­sium at West­mead Hospital.

“We’ve designed the test on two lev­els,” Dr Mina explained.

“Lev­el one we test for four para­me­ters – cre­a­ti­nine, pH, spe­cif­ic grav­i­ty, and oxi­dant assay – and they are basi­cal­ly test­ing for both sam­ple dilu­tion and some oth­er iterations.

“If we pick up any­thing, we go to sam­ple valid­i­ty test­ing lev­el two which is this new­ly devel­oped method in which we can analyse 23 dif­fer­ent para­me­ters that are always present in human urine and should be there in a sample.”

Two graphs showing a biological urine sample with many peaks indicating its components, versus a graph of a synthetic urine with far fewer peaks.
No com­par­i­son: the real thing ver­sus syn­thet­ic urine samples.

He says the method is already help­ing to detect cas­es where urine sam­ples have been tam­pered with to avoid drug detection.

“We had a case recent­ly where we found a par­ent drug in a urine sam­ple but not its metabo­lites. When the sam­ple was test­ed by the new method, it seemed to be water spiked with a drug and was not a bio­log­i­cal sample.

“So that’s telling us that some­one topped the water up with some drugs to show that they are tak­ing their med­ica­tion because we didn’t find the drug metabo­lites or any of the 23 mark­ers we iden­ti­fied for urine.”

Dr Mina says the test­ing is done at the same time as the sam­ple is analysed for more than 70 drugs so there is no loss of effi­cien­cy for the laboratory.

“In devel­op­ing this test we asked is it syn­thet­ic urine? Is it sub­sti­tut­ed urine? Is there an added adul­ter­ant to the urine? This method answers all those ques­tions, and we are the only lab in Aus­tralia, and pos­si­bly the world, cur­rent­ly able to do this.”

Dr Mina will also be pre­sent­ing his research at the upcom­ing IAFS con­fer­ence in Syd­ney in Novem­ber 2023.

Collaborating with a global technology leader to deliver better patient care

NSW Health has appointed health technology provider Epic to work with eHealth NSW and NSW Health Pathology to deliver a Single Digital Patient Record (SDPR) that will enhance the care patients receive at NSW public hospitals.

NSW Min­is­ter for Health the Hon. Ryan Park, MP announced the SDPR on 19 Octo­ber 2023, giv­ing the green light to uni­fy­ing health care tech­nolo­gies across NSW to pro­vide patients with improved hos­pi­tal care and pro­vide greater sup­port for busy health care providers.

Cre­at­ing a sin­gle statewide pathol­o­gy lab­o­ra­to­ry infor­ma­tion sys­tem is an impor­tant part of the SDPR that will pro­vide health pro­fes­sion­als with secure real-time access to pathol­o­gy results for their patients, no mat­ter which NSW pub­lic hos­pi­tal they are treat­ed in.

“This excit­ing trans­for­ma­tion pro­gram will improve patient care across NSW by enabling a high­ly secure, holis­tic and inte­grat­ed view of the care a patient receives across the NSW pub­lic health sys­tem,” said Vanes­sa Janis­sen, Chief Exec­u­tive of NSW Health Pathology.

“This means that if some­one from Dub­bo, for exam­ple, needs urgent hos­pi­tal care in Syd­ney, the treat­ing doc­tors will have imme­di­ate access to their med­ical his­to­ry and diag­nos­tic results and can make fast, informed deci­sions about their care”, she said.

SDPR will also enhance impor­tant dig­i­tal inte­gra­tions between our Foren­sic and Ana­lyt­i­cal Sci­ence Ser­vice, NSW Police, and the Coro­ner as well as across our pathol­o­gy net­work to help improve time­li­ness, informed deci­sion mak­ing and out­comes for the jus­tice system.

NSW Health Pathol­o­gy Chief Med­ical Infor­ma­tion Offi­cer Dr Stephen Braye said pathol­o­gy plays a vital role in the clin­i­cal diag­no­sis and treat­ment of patients and hav­ing this infor­ma­tion on hand fast is important.

“It’s why a sin­gle pathol­o­gy lab­o­ra­to­ry infor­ma­tion sys­tem is a core fea­ture of the SDPR ini­tia­tive – to ensure doc­tors can secure­ly access real-time pathol­o­gy results for patients no mat­ter which NSW pub­lic hos­pi­tal they’re being treat­ed in,” Dr Braye said.

Epic is a world leader in dig­i­tal health tech­nol­o­gy and will work with us to devel­op the SDPR, which will also include a sin­gle elec­tron­ic med­ical record (eMR) and one patient admin­is­tra­tion sys­tem (PAS).

Work on the design and build of the SDPR will soon begin and is sched­uled to go live at Hunter New Eng­land Local Health Dis­trict. It is then planned to be rolled out across the state in phases.

New Chief Executive at NSW Health Pathology

Australia’s largest public pathology provider and world-class forensic service, NSW Health Pathology has a new Chief Executive, with the appointment of Vanessa Janissen to the role.

A for­mer senior exec­u­tive at the organ­i­sa­tion, Ms Janis­sen brings a wealth of expe­ri­ence and a proven track record in pub­lic and pri­vate health services.

“I am excit­ed to be back at NSW Health Pathol­o­gy and am par­tic­u­lar­ly look­ing for­ward to guid­ing the organ­i­sa­tion through its next stage of evo­lu­tion, which will see us work close­ly with our lead­ers and high­ly skilled sci­en­tif­ic and med­ical teams to con­tin­ue to dri­ve improved out­comes and expe­ri­ences,” Ms Janis­sen said.

“Togeth­er we will be mak­ing the most of the advances in dig­i­tal, sci­ence and tech­nol­o­gy for our cus­tomers, part­ners and community.”

Ms Janissen’s arrival fol­lows the depar­ture ear­li­er this year of found­ing Chief Exec­u­tive Tracey McCosker PSM, who was appoint­ed Chief Exec­u­tive at Hunter New Eng­land Local Health District.

“I would like to pay trib­ute to the 10 years of ser­vice for­mer Chief Exec­u­tive Tracey McCosker gave to NSW Health Pathol­o­gy, over­see­ing its estab­lish­ment as a tru­ly statewide pathol­o­gy and foren­sic ser­vice in 2012.

“Her inspi­ra­tional lead­er­ship and patient-focused, strate­gic think­ing has helped cement NSW Health Pathol­o­gy as a trust­ed part­ner and leader in pathol­o­gy and forensics.”

“I also want to extend my thanks to Pro­fes­sor Rob Lin­de­man for act­ing in the Chief Exec­u­tive role for much of the year to date.

“Rob has ably led NSW Health Pathol­o­gy with his strong clin­i­cal exper­tise and lead­er­ship expe­ri­ence. I look for­ward to work­ing along­side him and the rest of the Strate­gic Lead­er­ship Team as we chart our next course.”

Ms Janis­sen held a num­ber of lead­er­ship posi­tions at Cal­vary Health­care, most recent­ly as the Nation­al Direc­tor, Strat­e­gy and Ser­vice Devel­op­ment lead­ing their growth strat­e­gy across hos­pi­tal, aged care, com­mu­ni­ty and vir­tu­al care services.

She was also NSW Health Pathology’s Exec­u­tive Direc­tor Strat­e­gy and Clin­i­cal Ser­vices Trans­for­ma­tion between 2013 and 2019. Pri­or to that she held mul­ti­ple oper­a­tional roles in Hunter New Eng­land Local Health District.

Her exper­tise in strat­e­gy, plan­ning, dig­i­tal trans­for­ma­tion, and ded­i­ca­tion to enhanc­ing human-cen­tered care will fur­ther strength­en NSW Health Pathol­o­gy’s posi­tion as a lead­ing pathol­o­gy and foren­sic ser­vice provider for the peo­ple of NSW.

“This organ­i­sa­tion tru­ly demon­strat­ed its exper­tise and val­ue as a con­nect­ed statewide net­work through­out the response to the COVID-19 pan­dem­ic when it played an instru­men­tal role in help­ing keep our com­mu­ni­ties safe.

“While this new era comes with its own chal­lenges, I’m con­fi­dent we will again rise to these, find­ing more new and inno­v­a­tive ways to enhance our ser­vices and the com­mu­ni­ties we serve.

“We will need to col­lab­o­rate even more close­ly with our part­ners in the health and jus­tice sys­tems to deliv­er more mod­ern, sus­tain­able ser­vices that meet the evolv­ing needs of our cus­tomers and communities.

“One of my ini­tial goals is to vis­it as many of our 5,000 plus staff in our 50 plus accred­it­ed lab­o­ra­to­ries, along with our key health and jus­tice part­ners, to bet­ter under­stand the chal­lenges and oppor­tu­ni­ties ahead so we can explore new and bet­ter ways of work­ing together.”

From emergency nursing to collections

Lee Wakeman has been a registered nurse blood collector for NSW Health Pathology for just over a year and is enjoying a welcome change of pace from her previous role in emergency nursing.

After nine years in nurs­ing, Lee Wake­man brings a wealth of expe­ri­ence to her role at NSW Health Pathology’s col­lec­tion ser­vice at Newcastle’s John Hunter Hospital.

“I worked in men­tal health and foren­sics as a new grad for the first 18 months of my career, where I gained expe­ri­ence that would help me tran­si­tion into my new role as an emer­gency nurse,” she said.

“I worked in emer­gency for six years, gain­ing lots of expe­ri­ence and many skills over the years, like triage and resus­ci­ta­tion skills.”

Lee says her work at NSW Health Pathol­o­gy allows her to col­lab­o­rate with doc­tors, oth­er nurs­es, and staff with­in the laboratory.

“My role now is to be a team leader and col­lect and coor­di­nate the pathol­o­gy col­lec­tions through­out John Hunter Hos­pi­tal, includ­ing the pae­di­atric wards,” she explains.

“I real­ly enjoy work­ing in pathol­o­gy, the hours are great, work­ing autonomous­ly and with­in a team, meet­ing lots of new peo­ple, mak­ing new friend­ships and you get the oppor­tu­ni­ty to get to know the patients.”

Lee also keeps her clin­i­cal skills up to date by work­ing casu­al­ly in a Post Anaes­the­sia Care Unit (PACU), oth­er­wise known as the recov­ery unit, in a local pri­vate hospital.

“I love that you can work in so many dif­fer­ent areas with­in nurs­ing and there is so many oppor­tu­ni­ties to learn and progress.”

Lee enjoys help­ing peo­ple and mak­ing a dif­fer­ence in their lives, no mat­ter how big or small, dur­ing a time that is dif­fi­cult in theirs.

“It’s a very dynam­ic and chal­leng­ing field to work in. But even the bad times have helped me improve my prac­tices and made me a bet­ter nurse.

“I have also made some of the best friends of my life whilst work­ing as a nurse.

“Out­side of work I love to trav­el, go out for nice food and wine, see my friends and family.”

Lee says she’s still learn­ing new skills all the time and would rec­om­mend a career in collections.

Keen to find out more? Check out the careers page on our web­site.

Take a look inside a compulsory drug treatment centre

Here at NSW Health Pathology we spend a lot of time analysing samples and producing results. Recently we got to see how our work is making a difference on the other side of a very high fence.

Inside a secure facil­i­ty on the grounds of Sydney’s Parklea Cor­rec­tion­al Cen­tre, two young men dressed in prison greens kick a foot­ball across a grassed court­yard. Anoth­er is busy in a kitchen prepar­ing chick­ens for roast­ing, and in a near­by art room a group of men are cre­at­ing extra­or­di­nary paintings.

But despite the razor wire on the fences, they’re not in prison; they’re part of a spe­cial drug treat­ment pro­gram over­seen by the NSW Drug Court.

Denise Con­stan­ti­nou is the Senior Psy­chol­o­gist at the Parklea Com­pul­so­ry Drug Treat­ment Cor­rec­tion­al Cen­tre and explains how par­tic­i­pants go through three stages of recov­ery after they arrive at the facility.

A woman smiling, sitting in an office.
Senior Psy­chol­o­gist Denise Con­stan­ti­nou works with par­tic­i­pants at the drug treat­ment centre.

“The pro­gram runs for a min­i­mum 18 months, with six months spent in each of the three stages,” she said.

“Stage one requires them to par­tic­i­pate in ther­a­peu­tic pro­grams to address their past drug use and offend­ing behaviour.

“We devel­op their treat­ment plan in col­lab­o­ra­tion with them and their adher­ence to that treat­ment plan is mon­i­tored on a week­ly basis.”

Ms Con­stan­ti­nou says par­tic­i­pants are required to pro­vide super­vised urine sam­ples reg­u­lar­ly through­out their treat­ment to ensure they remain drug free.

Cus­to­di­al offi­cers col­lect a urine sam­ple from par­tic­i­pants at least twice a week dur­ing stage one of the program.

“When they move to stage two the week­ly urine analy­sis actu­al­ly increas­es to three times a week because they’re able to access the com­mu­ni­ty,” she explains.

“Then in stage three when they are liv­ing out in the com­mu­ni­ty, but still part of the pro­gram, the urine analy­sis con­tin­ues at a rate of three times a week.

“All those tests hap­pen at the cen­tre so staff can see the par­tic­i­pants, assess their behav­iour, and ensure they’re not try­ing to evade detec­tion of drug use.”

Ms Con­stan­ti­nou says staff watch while the men give their urine sam­ple in a spe­cial­ly designed bathroom.

A open window looking into a room with a toilet and mirror on the opposite wall.
The design of the toi­let is aimed at pre­vent­ing par­tic­i­pants tam­per­ing with urine samples.
A large board covered in traditional Aboriginal dot designs and totems.
Dot paint­ings being cre­at­ed in the treat­ment cen­tre art room.

“Some will com­plain about hav­ing to par­tic­i­pate – there are some strin­gent pro­to­cols,” she said.

“We’re also very aware that some of our clients have expe­ri­enced a his­to­ry of trau­ma so uri­nat­ing in front of some­body can be very trig­ger­ing. So there needs to be some sen­si­tiv­i­ties around that process.

“But par­tic­i­pants also recog­nise that it does hold them account­able and ‘keeps them on track’. They know that if their drug use is detect­ed there will be con­se­quences in terms of the priv­i­leges and rewards that are afford­ed with­in the program.”

Ms Con­stan­ti­nou says some of the par­tic­i­pants are also work­ing on par­ent­ing plans to have con­tact with their chil­dren, so pro­vid­ing urine sam­ples means they can prove they’re stay­ing drug-free.

Sam­ples tak­en at the cen­tre are checked imme­di­ate­ly for drug mark­ers and if there’s a pos­i­tive indi­ca­tion, the urine is sent to NSW Health Pathology’s Drug Tox­i­col­o­gy Unit (DTU) for fur­ther test­ing and analy­sis to deter­mine what the drug is.

NSW Health Pathology’s Foren­sic & Ana­lyt­i­cal Sci­ence Ser­vice Direc­tor of Foren­sic and Envi­ron­men­tal Tox­i­col­o­gy San­ti­a­go Vazquez says the lat­est tech­nol­o­gy at the DTU lab­o­ra­to­ry ensures results are received by the Parklea cen­tre with­in days.

“We work with staff at the Com­pul­so­ry Drug Treat­ment Cor­rec­tion­al Cen­tre to ensure they know how to inter­pret the results of the urine tests and the reports that we pro­vide,” Dr Vazquez said.

“Our lab­o­ra­to­ry uses world-lead­ing tech­nol­o­gy to analyse a wide range of drugs and their metabolites.

“We’re proud to be sup­port­ing the work of the NSW Drug Courts and help­ing to man­age the recov­ery of participants.”

Since the drug treat­ment pro­gram began in 2006, the Parklea CDTCC has col­lect­ed more than 80,000 urine sam­ples for testing.

A woman in a Corrective Services uniform standing with another woman and a man outside a high-security fence.
Lin­da Fer­rett, Direc­tor Com­pul­so­ry Drug Treat­ment Cor­rec­tion­al Cen­tre, Dr San­ti­a­go Vazquez, NSWHP Foren­sic & Ana­lyt­i­cal Sci­ence Ser­vice, and Denise Con­stan­ti­nou, Senior Psy­chol­o­gist CDTCC.
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